The article aimed to investigate how different levels of nitrogen fertilizer, with the addition of humic acid and marine algae on the quantitative and qualitative traits of barley (Hordeum vulgare L.) grown in a semi-arid region of Iran, using a randomized complete block design with split-plots and four replication. The yield components of barley crop were significantly affected by all treatments involving organic matter compared to control. The maximum spike length, seed weight per spike, the number of seeds per spike, spike weight, biological yield, grain yield, grain phosphorus, grain potassium and grain zink were achieved using 140 Kg N ha-1.Application of Humic acid and seaweed extract has resulted in the highest value of spike length, peduncle length, seed weight per spike, the number of seeds per spike, spike weight, biological yield, grain yield, grain protein, grain phosphorus, grain potassium and grain zink. The increase in the yield components referred to the favorable effect of humic acid for improving early plant growth and increase dry matter accumulation in barley.The results presented in this study showed that organic matter of the recommended dose significantly increased grain yield and quality. Application of humic acid + seaweed extract along with 140 kg N ha-1 is necessary to achieve the highest performance of barley in semi-arid region of Iran.