Abstract: The aim of this study was to examine the effects of alpha lipoic acid (ALA) supplementation during asthenoteratozoospermia human semen cryopreservation on sperm quality, chromatin integrity and level of oxidative stress and expression of apoptosis related (BAX and BCL2), HSP70 and iNOS genes. Methods: The patients were infertile men with asthenoteratozoospermia. 20 freshly ejaculated semen samples were diluted, cryopreserved with Sperm Freeze Solution (SFS) that were supplemented with 0.00, 0.02, 0.05, 0.1, 0.5 and 1 mmol/ml of ALA. In order to find the optimal concentration of ALA in the sperm cryopreservation, samples were analyzed according to WHO (2010) before and after freezing. To determine the ALA effects on the molecular properties of sperm we assessed level of ROS production by flow cytometry, DNA fragmentation by TUNEL test and sperm cryo capacitation by CTC test and expression of stress protein (HSP70), pro-apoptotic (Bax), anti-apoptotic (Bcl-2) and iNOS genes by Real-time PCR for. Results: The effective concentrations of ALA (0.02 and 0.5 mM) significantly improved sperm motility and viability post–thawing compared to control group (treated with 0.00 ALA). During cryopreservation, treatment of semen with 0.02 mM of ALA (optimal concentration) significantly decreased DNA fragmentation, stress oxidative level (P<0.05), protected acrosome integrity and reduced BAX (no significantly) expression level while significantly increased BCL2, HSP70 and iNOS expression level in sperm cells compared to control group. Sperm quality was also improved in semen sample treated with 0.02 mM of ALA compared to control group, during cryopreservation. Conclusion: Our findings revealed that ALA can play a significant protective role against cryodamage by preserving sperm functional parameters. Keywords: Cryopreservation, Alpha lipoic acid, Chromatin integrity, Oxidative stress, Apoptosis, Asthenotspeeratozoormia