Precise determination of resistance by MIC assay is important in handling bacteria. Here we describe a protocol for identifying the MIC of B. agri C15 and B. agri C15 CdR with the hope of assisting researchers to correctly confirm the resistance of isolates .Its minimum inhibitory concentration (MIC) to Cd was 16 ± 0.7 mM and 21± 0.4 mM Cd, respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed no morphological effects of Cd on either of the strains. The projected assay to determine MIC of cadmium can be applied to the study of new agents with B. agri C15 and B. agri C15 CdR and in laboratory routine for other bacteria
The benefits of permanent His Bundle Pacing(p-HBP) resides in a physiological ventricular contraction. The development of specific tools that have made easier and faster the implant of the pacing lead in His opens the perspective of a more widespread use of this technique: our aim was to ascertain its long term reliability. Methods: Prospective cohort study including patients who undergone p-HBP with 12 months follow.up. We analyzed: success rate of the implantation, thresholds at follow-up, and complications related to pacemaker implantation at the baseline and after 12 months, including the mortality rates. Results: 72 patients(median age, 73(69-82)years) with a permanent pacing indication, in which p-HBP implantation was chosen as the first option. In 21.1% p-HBP was indicated with the purpose of cardiac resynchronization therapy, according to the heart failure guidelines. Implant success rate was 81.9%(n=59), with a median time of fluoroscopy of 6.5(3.4-13.5) minutes. The median acute His threshold was 1.5(1-2) Volts, at 24 hours 1.25(0.75-1.75) Volts and after 12 months 1.75(1-2.25) Volts. In the follow-up we registered a loss of capture in 5 cases(8.4%), with acceptable thresholds in 54 cases(91.6%). The total mortality rate in the follow-up was 4.2%(3 patients), for causes no related with the pacemaker implantation. Conclusions: p-HBP is a feasible and safe technique in a high percentage of patients, with reasonable fluoroscopy times, acceptable pacing thresholds acutely and at 1 year follow-up. Our results, even if obtained in a small sample, seems to be promising for the spread of this technique
The Subcarpathian area between Buzău and Slănic rivers, located in the south-eastern part of Romania, is one of the most affected areas by the torrential related phenomena. This occurs due to physical-geographical and economical-geographical factors, such as: slope, curvature profile, lithology, soil texture and land use. In order to highlight the influence of land use changes on the accelerated flow, these factors were integrated and worked in GIS environment. The land use changes were obtained and detailed from Corine Land Cover Data by applying and spatially modeling the binary change index and Markov model. By using GIS techniques, the above-mentioned factors were given bonitation scores, according to their influence on the surface runoff. After applying the methodology, mainly taken after Greg Smith (2003), the Flash-Flood Potential Index (FFPI) was obtained for the study area, with values between 10-22 during 1990-2006. During this period, the most important changes in the index values occurred in the central part of the study area.
Alzheimer’s disease (AD) is the most common type of dementia. Amyloid beta (β) oligomers are responsible for AD development and the evolution and aggregation of amyloid β oligomers is linked with insulin resistance which promotes cognitive decline and memory loss. Insulin resistance is also the major characteristic of type 2 diabetes mellitus (T2DM). Hyperglycemia, pancreatic β cell impairment, obesity, dyslipidemia and other comorbidities are linked to insulin resistance in T2DM. Hyperglycemia, neuronal loss, plaques and tangles are linked to insulin resistance in AD. Unhealthy lifestyle choices such as having a poor diet or not being sufficiently physically active can contribute to the development of AD and/or T2DM. Improving an unhealthy lifestyle, such as by doing aerobic exercise training (AET) can be useful in preventing or treating AD and T2DM. The present review deals with the similar mechanisms that underlie AD and T2DM, the beneficial effects of AET and highlights some of the mechanisms by which AET can induce positive changes in both pathologies.
Chrysopogon zizanioides (commonly known as vetiver) used in phytomanagement of metal polluted sites produces essential oil of industrial interest but the effect of metals on its volatile constituents is lesser known. The current study aimed to examine the quantity, quality and safety of essential oil, when vetiver plant was used for the phytomanagement of red mud. An experimental study was undertaken by growing the plant under varying red mud treatments viz. 0, 5, 10 and 15% w/w in sewage-sludge amended soil (soil:sludge:2:1 w/w) to appraise the effects on content and compositions of essential oil extracted from the roots. Garden soil without red mud and sewage-sludge served as a control and there were ten replicates of each treatment. Essential oil content in roots increased under all soil treatments except maximum red mud addition in sludge amended soil compared to the control. Gas chromatography and mass-spectroscopic analysis of vetiver oil revealed that 36, 35, 33 and 31 compounds were detected which contributed nearly 87.7, 90, 91.1 and 87% of total volatiles under 0, 5, 10 and 15% red mud treatments, respectively. Contents of major volatile constituents (rosifoliol, α-muurolol, farnesol, γ-costol, isovalencene and vetivone) increased, while selina-6-en-ol, cadin-4-en-10-ol, vetiselinenol and aristolone decreased with increasing red mud treatments. Contents of metals in essential oil were within the prescribed FSSAI limits for food. The study insinuate 5 and 10% (w/w) red mud utilization in soil amended with sewage-sludge for cultivation of vetiver to have improved quantity and quantity of essential oil, without any metal contamination possibility.\nKeywords: Chrysopogon zizanioides; essential oil; metals; red mud; sewage-sludge; volatile constituents.