Herein, preparation of chitosan/azelaic acid (CsAz) nanoparticles, loaded with lemon balm extract (LBE), and optimization of the formulation by using a mathematical model is reported. The model was designed by experimental design technique, to evaluate the effect of weight ratio of chitosan to azelaic acid (Cs/Az), tripolyphosphate (TPP) concentration and LBE weight on the loading efficiency of lemon balm in the chitosan nanoparticles. Detailed evaluations by the model indicated that the Cs/Az ratio (p-value 0.3014) exhibited no significant effect on the loading efficiency. Meanwhile, TPP concentration (p-values 0.0021) and LBE weight (p-value <0.0001) indicated dominant and significant influences, respectively, on the results. By increasing LBE weight and TPP concentration, the loading efficiency decreased. The optimum formulation to get maximum value of loading efficiency was determined as 2.00 (wt/wt) Cs/Az, 0.10 (mg/ml) TPP concentration and 68.48 (mg) LBE weight. The antimicrobial activity of the optimum sample was evaluated on ten different gram-positive/gram-negative bacteria or fungi. A synergistic effect was observed between azelaic acid and LBE. In some cases such as Enterococcus faecalis (ATCC 19433) the formulated system exhibited a better performance compared with neat azelaic acid or gentamicin.
Newcastle disease (ND) is one of the important avian viral diseases which is highly contagious and causes high rates of morbidity and mortality. The control of the disease requires accurate estimates of prevalence and risk factors of the disease. This study aimed to determine the molecular and serological prevalence of NDV and the risk factors associated with the seropositivity in backyard birds in Iran. The cross-sectional study has been performed in four provinces of Iran. A stratified random sampling method was used to select the villages. In each province at least eight villages were randomly sampled. In each village four families and from each family 12 birds were sampled. Serum samples and cloacal swabs collected from the birds. Information on associated risk factors was assessed using a structured questionnaire. Serum sampled underwent HI test and cloacal swabs were tested using the RT-PCR method. A logistic regression model was applied to assess the associated risk factors at the family level. The overall seroprevalence at bird, family and village level was 50.6% (95% CI: 48.1-53.1%), 93.4% (95%CI: 87.4- 96.7%), and 100.0% (95%CI: 91.2- 100.0%) respectively. According to the PCR test, NDV prevalence at family and village level was 29.4% (95%CI: 22.4-37.55) and 60% (95%CI: 44.6-73.6) respectively. None of the risk factors was associated with NDV seropositivity in the family. The results show that backyard chickens are highly exposed to NDV and we need to convey a fully covered program to control the disease in backyard chickens.
This research purpose to analyze the effect of implementation of 5S (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) to business performance in the manufacturing industries. This research uses a quantitative approach with Structural Equation Modeling (SEM) using smart PLS software. Data collection methods using online questionnaires and snowball sampling technique, the number of respondents to be used are 240 respondents of manufacturing managers in Indonesia . The results of this research conclude that Seiri has a positive and significant effect on the business performance, Seiton has a positive and significant effect on the business performance, Seiso has a positive and significant effect on the business performance, Seiketsu has a positive and significant effect on the business performance, Shitsuke has a positive and significant effect on the business performance. This novelty of this research is build a new model of the effect of applying Seiri, Seiton, Seiso, Seiketsu, and Shitsuke to the manufacturing industries in Indonesia.
This study aims to analyze the influence of Gemba Kaizen culture and implementation of 5S (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) to manufacturing performance in the manufacturing industries in Indonesia. This research uses a quantitative approach with Structural Equation Modeling (SEM) using smart PLS software. Data collection methods using online questionnaires and simple random sampling technique, the number of samples to be used as respondents as many as 300 manufacturing managers. The results of this study indicate that the gemba kaizen has a positive and significant effect on the manufacturing performance. For 5S also has a positive and significant effect on on manufacturing performance. The implication of this research is to be a reference that kaizen and 5S can be applied in several manufacturers to improve their performance and can be a reference to be applied in other industries . This novelty of this research is build a new model of the effect of applying new kaizen and 5S to the manufacturing industries in Indonesia.
This paper is an explanatory sequential mixed methods research aimed to explore and investigate the influence of halal food perception, halal food quality, halal food safety and halal food knowledge on purchase intention of consumers in South East Asia. The first stage is a quantitative analysis, the questionnaires were distributed randomly in by online electronic questionnaires and use snowball sampling technique, total of 340 respondents were filled online electronic questionnaires. The data processing and hypotheses which were tested using Structural Equation Model (SEM) Linear and Structural Model (LISREL), The next stage is a qualitative analysis exploring factors influence of non muslim consumers to buy halal food. Primary data collection using in-depth interviews with 5 (five) of consumers who were selected and determined using purposive sampling method Our analysis results indicated that halal food perception has a significant effect on purchase repetition , halal food quality has a significant effect on purchase repetition, halal food safety has significant positive influences on the purchase repetition and also halal food knowledge has a significant effect on purchase repetition. The result of this study may provide an insight to halal food producers to look into the South East Asian consumers as one of their target market in halal food industry and will help to know the level of South East Asian Consumers knowledge and perception toward halal food. Novelty of this research is a new model of halal food perception, halal food quality, halal food safety and halal food knowledge on purchase intentions of South East Asian consumers in Indonesia with the Explanatory Sequential Mixed Methods method with the scope of the territory of a country. This research can be a reference for similar research, and subsequent research can be conducted in other countries and could be adapted and adopted by other countries or in other regions.
Advancement in information and communication technology provides a scalable platform for various communication services, where a remote user can access the server from anywhere and anytime. The multi-server environment introduces a scalable platform such that a user can interact with any server using single registration. These services adopt authentication schemes in order to ensure secure and privacy preserving access to the resources. Recently Zhao et al. proposed a user authentication scheme based on password and smart card for the multi-server environment. They argued that their scheme is secure from well-known attacks. In this paper, we first identify that Zhao et al.’s scheme suffers from the denial of service attack and the privacy attack and does not provide user friendliness. After that, we propose a new privacy preserving user authentication scheme as a remedy scheme of Zhao et al.’s scheme. Through the formal and informal security analysis, we show that the proposed scheme is secure against various known attacks including attacks founded in Zhao et al.’s scheme. Furthermore, we provide the formal security verification of the proposed scheme using ProVerif tool.
Different methods have been used by far to prolong the shelf life of date fruit. However, 30% of all domestic date products are wasted, which calls for new ways of preservation. The present study was designed to use hurdle technology to produce and preserve the canned date. Canned date treatments were produced by a combination of three hurdles including pH, temperature and water activity. Values of pH, total soluble solids, texture firmness, microbial and sensory properties were measured over a span of six months. The level of pH and total soluble solids significantly increased for all the treatments (P<0.05). The microbial culture was negative in all cases. Time incurred no significant effects on sensory properties (P>0.05). The results indicated that canned date produced by hurdle technology and packaging had optimal microbial and sensory properties and can be used as an alternative to traditional methods of the date preservation.