This study aimed to consider the activity of immobilized α-glycosidase, significant enzyme defined in serum glucose adjustment, on Pluronic®F127 micelles and to compare the results with theoretical molecular docking studies. The phenolic molecules were used for the activity studies, which play a crucial role in the inhibition of α-glycosidase by providing conservation against pathogen factors. The immobilization of α-glycosidase on Pluronic®F127 micelles showed higher kinetic activity towards p-nitrophenyl-D-glucopyranoside (pNPG) than the free one. The inhibition profiles from some phenolic molecules were observed impressively with IC50 values between 20.47 - 1118.68 nM and Ki values between 18.51 - 1087.53 nM against immobilized α-glycosidase. In addition, the inhibition effects of the studied phenolic compounds have been tested with calculated negative binding energies from theoretical molecular docking studies. Negative binding energy values were as -6.1 and -8.3 kcalmol-1 for p-coumaric acid and tetrakis compounds, respectively. Experimental and theoretical findings were in good agreement.

The current study was designed to dissect the genetic diversity in agro-morphological traits as well as evaluate the endogenous potential of induced terminal heat tolerance on 205 Pakistani genotypes sown for two cropping seasons (2018-19 and 2019-20) with separate control in each year. Out of 21 morphological and yield-related traits PEL, NT/P, LA, Y/P, BY, HI, SSGW, G/S, and TGW showed a higher coefficient of variation (CV) in comparison to control, which was directly associated with the extent of variability in the subjected population. A strong positive correlation was observed among LA with FLL and FLW, DM with GFD, Y/P with HI, SSGW with TGW, G/S with SW in both growing year and control. However, BY showed a strong negative correlation with HI in both years and control. The first two axes of bi-plot, F1 and F2 of PC1 as well as PC2 explained 17% and 12% for the year 2018-19, 16% and 15% for the year 2019-20, as well as14% and 12% variability in control conditions respectively. Moreover 14 genotypes CB-42, CB-94, CB-176, CB-200, CB-208, CB-210, CB-239, CB-247, CB-350, CB-373, CB-399, CB-420, CB-484 and CB-498 showed a consistent behavior and positive correlation with yield and other variables of this group during both seasons and control year. Total of 8 genotypes CB-11, CB-19, CB-103, CB-216, CB-228, CB-373, CB-400 and CB-403 showed heat susceptibility index (HSI) of 0.23-0.34, 0.24-0.34, 0.30-0.37, 0.34-0.25, 0.21-0.32, 0.20-0.17, 0.42-0.37 and 0.45-0.31 and hence designated as (HSI≤ 0.5 ) highly heat-tolerant genotypes. Moreover, the above-listed genotypes also showed ≤ 20% cumulative decline in 1000-kernel weight in both seasons in comparison to control. Dissimilarity-based wards cluster analysis divided the population into three distinct clusters in both growing seasons and control respectively. Based on GLR analysis all genotypes (205) were divided into 4 categories i.e. 11% resistant (R), 48% moderately resistant (MR), 22% moderately susceptible (MS) and 19% susceptible (S), respectively.

A 6-year-old male Rottweiler dog presented with a marked weight loss combined with abdominal pain and diarrhea over 3 month. Abdominal ultrasound revealed a heteroechoic mid-abdominal mass with a focal hypoechoic area measuring 6.0 x 5.0 cm. Histopathology demonstrated massive lympho-proliferative lesions( low grade lymphoma). A one-month follow-up showed no-recurrence.

Plant cells store energy in the form of oil bodies constructed by structural proteins such as oleosins and caleosins. Although oil bodies usually accumulate in the seeds and pollen of plants, caleosins are ubiquitous and exist in the various organs and organelles. This issue, coupled with the diverse activities of caleosins, makes an ambiguity in the description of these oleo-proteins. Therefore, the current article proposes a new classification based on the bioinformatics analysis to characterize the basis of structure-function relationships of caleosins. Accordingly, four major structural groups were created by considering the transmembrane topology. The non-cytoplasmic sequences were the most abundant and diverse caleosins, especially in lower plants. Comparing the results with other reports suggests a stress response capacity for this caleosins. However, the other three groups play a more specific role in germination and pollination. Finally, a phylogenetic tree was built consisting of two main clades. The clades showed a significant difference in molecular weight, isoelectric point, caleosin group, and expression profile (P<0.01). In addition to the physiological significance of these findings, predicting the structure of caleosins is necessary for constructing oil bodies used in the food and pharmaceutical industries.

Occupational health and safety implementation, compliance and management has continued to take the center stage in the decision-making process in most organisations. This view is supported by the fact that the South African Department of Labour and government intervenes through legislation to promote safer work environment. The increased emphasis on occupational health and safety in the manufacturing industry in recent years necessitates continuous research on management of health and safety at workplaces, the motive behind the implementation of health and safety practice in the manufacturing sector has been explored to a limited extent. \nThe aim of the study is to assess how organisations can increase productivity through the implementation of OHSA in Air Products Company. In order to achieve this objective, the study investigated the level of implementation, compliance and awareness amongst employers and their employees concerning safety and health matters. Furthermore, the study also discussed the level of safety culture and employees’ attitude concerning the underlying principles of the Occupational Health and Safety Act (OHSA) of 1994 in South Africa, that promotes self-regulation and monthly checklists at workplaces. The study investigates the prevalence of implementation and compliance to occupational health and safety requirements through using a questionnaire survey and interviews. The findings reveal conflicting responses between both management and subordinates and this is crucial in understanding health and safety practices and their effectiveness on employees’ performance for the success of the organisation.

Recently, many investigations on anthocyanins have unraveled their strong antioxidant activity and their possible use as chemotherapeutics. \nIn this work, we report the preliminary results of the induction of anthocyanin biosynthesis in sour cherry in Iran’s forests through metal ions chelation of some anthocyanins [ACN- Al3+/Ga3+/Cr3+ /Fe3+ /Mg2+ ] chelation in aqueous medium were investigated to discover the color fluctuation of different derivatives of these compounds in the low pH. Chelation of Mn+ by ACN also improved the color expression range of ACN in more different pH environments.\nIn this verdict, it has been studied the metal ions diffusing of deprotonating for the ACN B-ring of Cyanidin, Delphinidin and Petunidin in vacuum and water media, transforming flavylium cations to the blue quinonoidal bases at lower pH using the infrared method though pursuing Beer-Lambert law for finding the physical and chemical properties of frequency, intensity, and absorbance of the compounds, respectively in Iranian sour cherry. In this wok, it has been illustrated that the important factor for increasing the absorbance in a positive non-linear fashion due to deviating from the Beer-Lambert law is the self-association of anthocyanins of Cy, Dp and Pt anthocyanins.\nThe difference of ∆HR among [ACN- Al3+/Ga3+/Cr3+/Fe3+/Mg2+] chelation complexes has been discussed the double bonds and carbonyl groups through the chelation of B ring for Cy, Dp and Pt anthocyanins in vacuum and water media that explains the stability and color of [ACN- Al3+/Ga3+/Cr3+/Fe3+/Mg2+] chelation of Cy, Dp, and Pt pigments in a weak acidic condition in Iranian sour cherry.\nThe ACNs of Cy,Dp and Pt with highest chelate in the active part of these structures by metal ions of Al3+/Ga3+/Cr3+/Fe3+/Mg2+ produce a variety range of colors under acidic pH; as divalent Mg2+ has shown various physicochemical properties considering its optimized geometry optimization as the bond length of O Mg2+ ≈1.9 Å and the bond angle of O Mg2+ O ≈ 112o while it has been indicated the bond length of O M3+ ≈1.8 Å and bond angle of O M3+ O ≈ 120o for trivalent metal cations of Al3+/Ga3+/Cr3+/Fe3+/Mg2+ with more electron rich metal ions caused shifts and hue changes in the stability of color and structure. \nMoreover, we have found that [ACN- Al3+/Ga3+/Cr3+/Fe3+/Mg2+] chelation is dependent on the position of active cites of labeled oxygen atoms and cations in these structures which transfer the charge of electrons in aromatic cyclic chains because of water polar medium in contrast to vacuum. The spin density and partial charges have been obtained by fitting the electrostatic potential to fixed charge of O+17 , O+16 , and O+7 cations for Cy-Mn+(31), Dp-Mn+ (32) and Pt-Mn+(35) , respectively based on the electrophilic groups of Cy, Dp and Pt anthocyanin pigments which unravel the activity and the stability of these compounds in the natural products like Iranian sour cherry. Then, Monte Carlo simulation unravel the logic agreement results through calculation of the relation confidents in the electrophilic structures; R² cyanidin= 0.8237, R² delphinicin= 0.8964 and, R² petunidin = 0.9208.