A series of novel benzo[1,2,3]selenadiazole-isoxazole hybrid compounds 11a-e were designed and hemisynthesized from natural (R)-carvone 8. These derivatives were synthesized via a selenation reaction between selenium dioxide (SeO2) and semicarbazones 10a-e themselves prepared from naturally occurred (R)-carvone 8 using successively, 1,3-dipolar cycloaddition and semicarbazone condensation reactions. The structures of the newly synthesized products were fully characterized by spectroscopic analysis (1H, 13C NMR) and HRMS. All the synthesized compounds were tested in vitro against four human cancer cell lines, fibrosarcoma (HT-1080), lung (A-549) and breast (MCF-7 and MDA-MB-231) carcinoma to evaluate their anticancer activity. Most of the semicarbazones 10a-e and some of the benzo[1,2,3]selenadiazole-isoxazole hybrids 11a-e showed interesting cell growth inhibitory activity with IC50 values ranging from 10 to 20 µM. Furthermore, compounds 10a and 11c exhibited significant anti-proliferative activity against HT-1080 cells with IC50 values close to 10.94 ± 1.21 and 18.68 ± 2.62 μM respectively.

Wheat is considered among non-accumulator but one of the Se tolerant plants. Wheat seedlings were hydroponically grown in hoagland solution and treated with various level of Se. Tolerance response to Se toxicity was investigated by determining the level of thiobarbituric acid reactive substance (TBARS), proline and chlorophyll content, the growth parameters, and the activity of several antioxidant enzymes. Toxic level of Se treatment significantly retarded the seedling growth. Substantial amount of proline accumulation was also observed in response to toxic Se concentration, but it was more pronounced in putative sensitive cultivar. Chlorophyll content was significantly decreased in Se intoxicated seedlings and increased in the lowest Se dose. A severe and mild chlorosis was observed at the highest Se level in putative sensitive and tolerant cultivars, respectively. Alterations in the activities of glutathione reductase (GR, 1.6.4.2), glutathione S transferase (GST, 2.5.1.18), guaiacol peroxidase (GPX, 1.11.1.7), catalase (CAT, 1.11.1.6), and ascorbate peroxidase (APX, 1.11.1.11) and superoxide dismutase (SOD, EC 1.15.1.1) were determined. TBAR level, as indicator of membrane lipid peroxidation, was not significantly increased in putative tolerant cultivar although a significant increase was observed in putative sensitive cultivar in response to higher selenium concentrations in growth media. Activities of CAT, and SOD were significantly increased in putative Se tolerant cultivar at higher Se treatment groups. However, excluding SOD, activity of all the studied enzymes was significantly increased in putative sensitive cultivar. Higher antioxidant enzyme activities and substantial amount of proline accumulation did not provide significant contribution to overcome Se phytotoxicity in wheat seedlings grown in media supplemented with toxic level of selenium.

ABSTRACT\nAim: Individuals with psychiatric symptoms do not get enough help and anxiety and depression are known problems for oncological patients. However, the psychiatric status and treatment of patients who routinely present to nuclear medicine clinics for diagnosis, staging and for treatment response are not well documented. In this study, the psychiatric symptom profiles and help-seeking behaviours of oncological patients referred to a nuclear medicine clinic were examined.\nMethod: Each oncological patient who presented to the nuclear medicine clinic filled out a sociodemographic data form, a psychiatric admission evaluation form for oncology patients, a Beck Anxiety Inventory (BAI), a Beck Depression Inventory (BDI) and a Symptom Checklist-90 (SCL-90) form for psychiatric symptom screening.\nResults: Beck Depression Total and Beck Anxiety Total values were found to be significantly higher in women than in men. Whereas 27.8% of the patients had a Beck Depression score ≥18 and were in the risk group for depression, 31.7% of the patients experienced moderate or severe anxiety symptoms. But $.5 of the patients had help seeking experience during their whole life, .2 seeked psychiatric help during last 6 months, .4 seeked psychiatric help after cancer diagnosis and .3 ha a psychiatric treatment (medication) at the moment. The rate for psychiatric medication in high-risk group for depression was % and for anxiety was 26.9%. \n\nConclusion: The attention and guidance of physicians surrounding the psychiatric symptoms and help-seeking behaviours of oncological patients who present to nuclear medicine clinics for FDG-PET/CT imaging are very important factors in psycho-oncology.

The study intended to determine the proximate composition, caloric value, carbohydrate profile and selected minerals of the following cereal crops: Pigmented rice (Oryza sativa L.) varieties (waxy-type purple rice ‘Ballatinao’ (PR) and non-waxy type red rice ‘Malagaya tapol’ (RR)), non-pigmented rice varieties (waxy type ‘Malagkit Songsong’ (MS) and non-waxy type ‘IR-64’), alday (Coix lachryma-Jobi L.) and Obatanpa x Lagkitan (OxL) variety corn (Zea mays L. ‘Los Baños Lagkitan’). Results revealed that all cereal crops exhibited a low moisture content (3.00-4.16%) which makes them store for a long time. IR-64 had the highest fat content of 4.76%, significant (p<0.05) to the values exhibited by adlay and OxL, but not significant (p>0.05) to the values of RR, PR and MS. It was also observed that adlay had the highest values for protein (11.94%), total ash (1.64%) and iron (1.59mg/100g). On the other hand, MS recorded the highest amount of fiber (3.40%) and RR contained the highest amount of zinc (1.76mg/100g) and caloric value (399.85 kcal/100g). OxL exhibited the highest carbohydrates among the samples. Carbohydrate profile analysis revealed that all rice varieties showed the highest amount (p<0.05) of starch content (84.45-85.45%) while adlay and MS recorded the highest amylose content (26.18%) and amylopectin content (76.63%), respectively. Overall, these cereal crops were shown to be rich in carbohydrates, proteins, and minerals justifying their use in diets and potential use for future product development.

This research focuses on the health risks caused by heavy metals (HMs) environmental pollution. Soil, water, corn, rice and patients\' hair samples from Daping Village, Yunnan Province, China were analyzed for seven selected HMs. Geoaccumulation index (Igeo), pollution indexes (PI), and the Nemerow integrated pollution index (PN) were used to evaluate pollution levels. We employed principal component analysis (PCA), correlation analysis (CA), and spatial distribution to identify the source and distribution characteristics of HMs in soil. Health risks of HMs and exposure pathways were accessed by calculating the Hazard Quotient (HQ) and Hazard Index (HI). The Igeo, PI, and PN results show that Cadmium (Cd) and Arsenic (As) pollution is severe in soil, while other pollution is relatively little. PCA, CA, and spatial distribution show that HMs may be derived from black shale weathering and enrichment. Residents’ drinking water is relatively safe. Arsenic is the element most threatening to local residents (HI=3.8). Soil (HI=3.55) and plant (HI=1.67) ingestion are the primary exposure pathways to HMs. This unusual disease may be caused by children’s relatively low immunity and long-term exposure to As. We must enhance the protection of children and encourage avoiding soil contact as much as possible. Our results highlight the importance of investigating HMs pollution from geological sources and blocking potential exposure pathways.

Background and Aims: To investigate the genomic signatures and prognosis of advanced-stage T cell lymphoblastic lymphoma (T-LBL) and examine the relationship between T-LBL and T cell acute lymphoblastic leukemia (T-ALL). Methods: A total of 35 Chinese T-LBL children with stage III or IV disease were recruited in this study. They were treated with combination chemotherapy, while whole-exome sequencing was performed in these patients. The relationship of the clinical features, prognosis, and specific gene mutations in the cohort was researched. Gene chips of T-LBL and T-ALL were downloaded from a database, and differential gene expression was analyzed. Results: Germline causal gene mutations (CARS or MAP2K2) were detected in 2 patients without a family history of cancer; 3.06±2.21 somatic causal gene mutations were identified in the 35 patients, and somatic mutations were observed in the NOTCH1, FBXW7, PHF6, and JAK3 genes. NOTCH1 mutations were significantly associated with FBXW7 mutations (6/35, 17.14%), and the age at diagnosis of patients with NOTCH1-FBXW7 mutations was younger than that of patients without such mutations (P<0.05). One patient died of sepsis, and treatment-related mortality was 2.86%. Two patients were classified as progressive disease and quit this study, 32 achieved complete remission (CR) for a CR rate of 91.43%, and 14 and 18 patients were classified into the intermediate-risk (IR) group and high risk (HR) group. During a median follow-up of 44 months, three patients relapsed, and the relapse rate was 8.57%. Three-year prospective event-free survival (pEFS) was 82.286%, and no significant differences of pEFS were found for different sexes, ages, or statuses of NOTCH1-FBXW7 mutations (P>0.05); however, the mean survival time of the IR group was longer than that of the HR group (P<0.05). Differential expression of genes in the T-LBL and/or T-ALL datasets was analyzed using the R package limma, and 1/3 of the differentially expressed genes were found in both the T-ALL and T-LBL datasets. High expression of FI3K-Akt signal pathway genes and the USP34 gene was found in the T-LBL dataset. Conclusion: Although T-ALL and T-LBL both originate from precursor T-cells and are considered different manifestations of the same disease and the outcome of T-LBL is favorable when using T-ALL-based chemotherapy, there are differences in the gene distribution between T-LBL and T-ALL. It seems that the FI3K-Akt signaling pathway and the USP34 gene play essential roles in T-LBL, but medicines targeting the USP34 gene or the FI3K-Akt pathway may be invalid