The fishes of genus Alburnus (Rafinesque, 1820) have no commercial value, which is probably why its biology and, particularly, taxonomic status has not attracted much interest. Seven valid species of genus Alburnus are known in Iran, namely A. chalcoides, A. filippii, A. atropatenae, A. caeruleus, A. zagrosensis, A. mossulensis and A. hohenackeri. Because of wide distribution of the genus in Iran, its taxonomic status needs urgentmore detailed studies. The present study was intended to have some contributions to the knowledge of poorly known morphology of this genus, in Iran. In order to compare morphometric and meristic characteristics of the genus, all the seven species were caught during the year 2010-2012 by electrofishing. Twenty-eight morphometric measurements, together with ten meristic traits, were used in the analyses including: Univariate analysis of variance (ANOVA) and multivariate sense, principal component analysis (PCA), discriminant function analysis (DFA), Canonical discrimination analysis (CVA) and cluster analysis (CA). The ANOVA revealed significant differences in 28 morphometric and 5 meristic factors (P<0.05). Principal component analysis (PCA) results from morphometric and meristic data also indicated that all specimens had 74.289% changes in 8 factors of the first morphometric characters and 85.953% changes in 3 factors of the first meristic characters. Plotting the first and second principal components of meristic characters showed that A. atropatenae and A. filippii had high degree of overlap with together, while another species had thorough separation from each other. In discriminant function analysis (DFA), the proportion of individuals correctly classified into their original groups was 97.4% and 94.1% for morphometric and meristic characteristics, respectively. The results of the present study suggest that all sampling sites represent independent specieson their own. The present study provides basic information about the differentiation of Alburnus species from different localities in Iran.