| DOI LINK: https://doi.org/10.59879/e9O5w |
| Paper ID:e9O5w |
| Volume:97 |
| Issue:1 |
| Title:The Potential Effect of Avocado Seeds to Promote Bone Formation of Tooth Extraction Sockets in Diabetic Rats. |
| Abstract:Objective: There is a significant difference in post-extraction complications in diabetes mellitus (DM) patients as this condition can influence each phase of wound healing due to being delayed. Avocado seeds (Persea americana Mill.) contain many compounds for instance polyphenol catechin compounds, including catechin, cyanidin, chlorogenic acid and quinic acid that can accelerate bone formation after tooth extraction through various mechanism. Bone formation involve RUNX2 as differentiation factor and proliferation factor for osteoblast. and Osteoblast plays and important role and involves in the formation of woven bone. The purpose of this research is to prove the potential of avocado seed extracts as a natural intervention to accelerate bone healing in diabetic conditions by promoting anti-inflammatory and antioxidant activities, thereby improving bone formation. Materials and methods: This study is an experimental research with posttest-only control group design. The research samples were 20 white male Rattus norvegicus and were divided into two groups: control (K) and treated (KE). Then, the lower left incicives of the samples were extracted for immunohistochemical staining to observe the expression of RUNX2, and H&E staining to study osteoblast and woven bone at day 7 and 14. Data was analyzed using the SPSS Mann-Whitney and Spearman test. p < 0.05 was considered statistically significant. Results: The results show significant differences between the control and treatment groups. The expression of RUNX2 and the number of osteoblasts were significantly higher in the treatment group. Additionally, there were significant correlations between RUNX2 expression, osteoblast count, and woven bone formation. This suggests that RUNX2 can enhance woven bone formation. The woven bone formed will eventually be replaced by mature bone. Conclusion: Avocado seeds have potential effect to accelerate bone formation in diabetic conditions by increasing the expression of RUNX2, osteoblast count, and woven bone formation. |
| Keywords:Avocado Seed, Bone Formation, Tooth Extraction, Diabetic. |
| Authors:Puspa Dila Rohmaniar, Retno Pudji Rahayu*, Ida Bagus Narmada, Siswandono, Agung Krismariono, David Buntoro Kamadjaja, Ninuk Hariyani, Faezah Rokhani, Anisa Ramadhani Kusumastiti, Nikmatus Sa`adah |
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